Suppression of lipopolysaccharide‐ and tumour necrosis factor‐α‐induced interleukin (IL)‐8 expression by glucocorticoids involves changes in IL‐8 promoter acetylation | Zendy

Tsaprouni L. G. | Zendy; Ito K. | Zendy; Adcock I. M. | Zendy; Punchard N. | Zendy

Open Access

Suppression of lipopolysaccharide‐ and tumour necrosis factor‐α‐induced interleukin (IL)‐8 expression by glucocorticoids involves changes in IL‐8 promoter acetylation

Author(s) -

Tsaprouni L. G.,

Ito K.,

Adcock I. M.,

Punchard N.

Publication year - 2007

Publication title -

clinical & experimental immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.329

H-Index - 135

eISSN - 1365-2249

pISSN - 0009-9104

DOI - 10.1111/j.1365-2249.2007.03484.x

Subject(s) - trichostatin a , acetylation , proinflammatory cytokine , tumor necrosis factor alpha , histone deacetylase , lipopolysaccharide , interleukin , microbiology and biotechnology , biology , cell culture , cytokine , endocrinology , immunology , medicine , chemistry , histone , inflammation , biochemistry , gene , genetics

Summary There is accumulating evidence that the transrepressional effect of glucocorticoids in down‐regulating proinflammatory gene expression might be regulated by an action on histone acetylation. To investigate this, we studied the effect of two glucocorticoids (dexamethasone and triamcinolone acetonide) on reducing lipopolysaccharide (LPS)‐ and tumour necrosis factor (TNF)‐α‐induced interleukin (IL)‐8 release in a monocytic cell line and two lymphocytic cell lines (HUT‐78 and Jurkat). The effect of the histone deacetylase inhibitor trichostatin A (TSA) on LPS‐ and TNF‐α‐induced IL‐8 release and its repression by glucocorticoids was also examined. LPS and TNF‐α induced IL‐8 release in all three cell lines and this induction was inhibited by both dexamethasone and triamcinolone. Pretreatment of cells with TSA enhanced basal and LPS‐ and TNFα‐stimulated IL‐8 release in all three cell lines. TSA also attenuated the inhibitory effect of glucocorticoids on stimulated IL‐8 release. Chromatin immunoprecipitation assays confirmed that LPS and TNF‐α enhanced histone acetylation at the IL‐8 promoter and that this was inhibited by triamcinolone in all three cell types. Changes in histone acetylation at the IL‐8 are important in its regulation by proinflammatory and anti‐inflammatory agents, and modulation of this activity may have therapeutic potential in inflammatory conditions.

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