Screening of a HUAEC cDNA library identifies actin as a candidate autoantigen associated with carotid atherosclerosis

SUMMARY The humoral immune response to endothelium has a pivotal role in the development of atherosclerosis. Using a molecular method, we sought to identify endothelial autoantigens in carotid atherosclerosis. Immunoscreening of a HUAEC expression library with IgG from a pool of two sera from patients with carotid atherosclerosis identified a clone specific to actin. We evaluated actin‐specific IgG reactivity in patients with carotid atherosclerosis and compared responses with those in patients with systemic lupus erythematosus and type 1 diabetes mellitus and in healthy subjects. Enzyme‐linked immunoassay detected actin‐specific IgG in a significantly higher percentage of sera from patients with atherosclerosis and systemic lupus erythematosus than from healthy subjects (16/61, 26% and 13/33, 39% versus 2/41, 5%, P  = 0·012 and P  < 10 −4 , by χ 2 test). Mean optical density values were significantly higher in patients than in healthy subjects ( P  < 10 −4 by Student's t ‐test). Patients with atherosclerosis and uncomplicated plaques had significantly higher serum anti‐actin IgG reactivity than those with complicated plaques ( P  = 0·048 by Student's t ‐test). Our findings suggest that actin is an autoantigenic molecule of potential clinical interest in carotid atherosclerosis.