Constitutive expression of Interleukin‐18 and Interleukin‐18 receptor mRNA in tumour derived human B‐cell lines | Zendy

LOREY S. L. | Zendy; HUANG Y. C. | Zendy; SHARMA V. | Zendy

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Constitutive expression of Interleukin‐18 and Interleukin‐18 receptor mRNA in tumour derived human B‐cell lines

Author(s) -

LOREY S. L.,

HUANG Y. C.,

SHARMA V.

Publication year - 2004

Publication title -

clinical & experimental immunology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.329

H-Index - 135

eISSN - 1365-2249

pISSN - 0009-9104

DOI - 10.1111/j.1365-2249.2004.02465.x

Subject(s) - biology , paracrine signalling , cytokine , secretion , immune system , messenger rna , receptor , interleukin , autocrine signalling , microbiology and biotechnology , interleukin 33 , cell culture , interleukin 15 , beta (programming language) , interleukin 4 receptor , immunology , gene , endocrinology , genetics , computer science , programming language

SUMMARY Interleukin‐18 (IL‐18) is a pro‐inflammatory cytokine involved in the Th1 immune response and expressed by a variety of cell types. IL‐18 is a member of the IL‐1 family and plays an important role in autoimmune diseases and inflammation. Using reverse transcriptase‐polymerase chain reaction (RT‐PCR) mRNA expression of IL‐18, IL‐18 receptor alpha (IL‐18R α ), and beta (IL‐18R β ) were studied in tumour derived human B‐cell lines. Furthermore, we investigated IL‐18 protein secretion by using enzyme linked immunosorbent assay (ELISA). The results, as presented in this report, suggest that IL‐18, IL‐18R α , and IL‐18R β mRNA are constitutively and ubiquitously expressed in human B‐cell lines, but secretion of the functional protein does not occur. We therefore speculate that IL‐18 possibly affects B‐cells through paracrine actions.

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