Open AccessProduction of immunoglobulins by human sIgD + and sIgD − human blood B lymphocytes in response to stimulation with activated T cells and agonistic antibodies; effect of IL‐10, IL‐2 and mode of activation of T cells
Author(s) -
LING N. R.,
BROWN B.,
HARDIE D.
Publication year - 1995
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1995.tb08366.x
Subject(s) - immunoglobulin d , biology , immunoglobulin class switching , cd40 , immunology , isotype , antibody , immunoglobulin g , immunoglobulin e , immunoglobulin m , b cell , interleukin 4 , microbiology and biotechnology , immune system , in vitro , monoclonal antibody , cytotoxic t cell , biochemistry
SUMMARY Production of IgM, IgG and IgA was induced from human blood B lymphocytes by culturing with a CD40 MoAb and IL‐2 for 9 days. Replacement of IL‐2 by IL‐10 markedly enhanced production of all three isotypes. High levels of immunoglobulin production also occurred when activated irradiated autologous T cells replaced the CD40 MoAb, and when IL‐10 replaced IL‐2 in these cultures a spectacular increase in IgG production occurred. The effectiveness of the T cell stimulus depended on the mode of purification of the T cells and the nature of the stimulant used to activate them. Differences in the kinetics and level of expression of CD40L on the various T cell preparations were observed, but did not account for variations in immunoglobulin‐inducing efficiency. Immunoglobulin production from sIgD + and sIgD − B cells was investigated. IgG and IgA were found in sIgD + cultures, indicating that some isotype switching had occurred, but the major part of the IgG and IgA secreted was from cells already committed to these isotypes. Anti‐IgD or anti‐IgM MoAbs enhanced the proliferation of B cells induced by anti‐CD40 antibody, but immunoglobulin production was not enhanced. Factors affecting the balance of proliferation and differentiation are discussed.