Open AccessExpression of IL‐10, IL‐4 and interferon‐gamma in unstimulated and mitogen‐stimulated peripheral blood lymphocytes from HIV‐seropositive patients
Author(s) -
DIAZMITOMA F.,
KUMAR A.,
KARIMI S.,
KRYWORUCHKO M.,
DAFTARIAN M. P.,
CREERY W. D.,
FILION L. G,
CAMERON W.
Publication year - 1995
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1995.tb06632.x
Subject(s) - immunology , stimulation , immune system , reverse transcriptase , biology , interferon , interferon gamma , cytokine , pathogenesis , interleukin 4 , in vitro , messenger rna , microbiology and biotechnology , endocrinology , polymerase chain reaction , gene , biochemistry
SUMMARY Infection of immune cells with HIV induces dysregulation of cytokines which may play a vital role in HIV pathogenesis. We analysed the expression of T helper type I (Th1) (interferon‐gamma (IFN‐γ)) and Th2 (IL‐4, IL‐10) type cytokines in peripheral blood lymphocytes (PBL) from HIV patients. The semiquantitative reverse transcriptase polymerase chain reaction (RT‐PCR) analysis revealed that IFN‐γ mRNA in unstimulated PBL was significantly decreased and IL‐10 mRNA was significantly upregulated in patients with < 400 CD4 + T cells/mm 3 ( n = 30) as compared to patients with > 400 CD4 + T cells/mm 3 ( n = 6) and normal controls ( n = 16). In addition. IL‐10 mRNA levels were inversely associated with IFN‐γ expression. Similar results were obtained by measuring IL‐10 production in the supernatants of PBL cultured in vitro without stimulation by employing an enzyme immunosorbent assay (ELISA). However, the levels of IL‐4 and IFN‐7 produced by unstimulated PBL were undetectable by ELISA. Mitogen stimulation of PBL revealed two groups of HIV individuals based on IL‐10 production. PBL from one set of individuals produced low levels of IL‐10 (low IL‐10 producers) whereas the other group produced IL‐10 comparable lo that of normal controls (IL‐10 producers). Production of IL‐4 was significantly reduced in HIV+ individuals with<400 CD4 + T cells/mm 3 as compared to the normal controls. However, ability to produce IFN‐γ by mitogen‐stimulated total PBL and CD4 + purified cells was not impaired in HIV + individuals. These results suggest that unstimulaied and mitogen‐stimulated PBL of HIV + individuals exhibit dysregulation of Th2 type cytokines which may play a role in HIV immunopathogenesis.