Open AccessAutoproliferation in HIV‐1‐infected patients undergoing active HIV‐1‐specific immunotherapy
Author(s) -
TRAUGER R. J.,
GIERMAKOWSKA W.,
WORMSLEY S.,
TURNER J.,
JENSEN F. C.,
CARLO D. J.
Publication year - 1995
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1995.tb03596.x
Subject(s) - immunogen , immunology , immunophenotyping , peripheral blood mononuclear cell , adjuvant , asymptomatic , cd8 , antigen , medicine , cellular immunity , immunotherapy , immunity , hiv antigens , virology , immune system , biology , human immunodeficiency virus (hiv) , antibody , viral disease , in vitro , monoclonal antibody , biochemistry
SUMMARY We have observed a treatment‐associated autoproliferative response in cultured peripheral blood mononuclear cells (PBMC) of asymptomatic HIV‐1‐infected subjects receiving a gp120‐depleted, inactivated HIV‐I antigen in incomplete Freund's adjuvant (IFA; HIV‐I Immunogen). The frequency and magnitude of the autoproliferative response appeared to be dose‐related (P<0.05), and was not observed in subjects receiving IFA alone. Immunophenotyping of the proliferating cells demonstrated the presence of both CD4 + and CD8 + lymphocytes, with the CD4 + blasts almost exclusively expressing the CD45RO + phenotype. A comparison of this response with the HIV‐1‐specific antigen stimulation responses in this cohort revealed a significant correlation between increases in HIV‐1‐specific cell‐mediated immunity and autoproliferation ( r 2 = 0.61, P < 0.001). These findings suggest that immunization with the HIV‐1 Immunogen induces an autoproliferative response that may reflect changes in HIV‐1‐specific cell‐mediated immunity in infected individuals.