Open AccessEffect of IL‐4 and interferon‐gamma (IFN‐γ) on IL‐3‐ and IL‐5‐induced eosinophil differentiation from human cord blood mononuclear cells
Author(s) -
OCHIAI K.,
IWAMOTO I.,
TAKAHASHI H.,
YOSHIDA S.,
TOMIOKA H.,
YOSHIDA S.
Publication year - 1995
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1995.tb03482.x
Subject(s) - eosinophil , peripheral blood mononuclear cell , immunology , interleukin 5 , interferon gamma , cord blood , umbilical cord , interleukin 4 , allergic inflammation , interleukin , biology , cytokine , inflammation , in vitro , biochemistry , asthma
SUMMARY IL‐4 and IFN‐γ positively and negatively regulate allergic inflammation. To determine the regulatory mechanisms of eosinopoiesis by cytokines, we examined the effect of recombinant IL‐4 and IFN‐γ and of anti‐IL‐4 and anti‐IFN‐γ antibodies on IL‐3‐ and IL‐5‐induced eosinophil differentiation from human umbilical cord blood mononuclear cells. rhIL‐4 (10–300 U/ml) inhibited IL‐3‐ and IL‐5‐induced eosinophil differentiation from cord blood mononuclear cells on day 28 of culture by 62–81% in a concentration‐dependent manner. rhIFN‐γ (5–500 U/ml) also inhibited IL‐3‐ and IL‐5‐induced eosinophil differentiation by 80–99% in a concentration‐dependent manner. The inhibitory effect of rhIL‐4 and rhIFN‐γ was observed only when rhIL‐4 or rhIFN‐γ were present in the culture from day 0 to day 14, but not from day 15 to day 28. Addition of anti‐IL‐4 antibody to the culture enhanced IL‐3‐ and IL‐5‐induced eosinophil differentiation on day 28 of culture by 30%, whereas anti‐IL‐2 MoAb and anti‐IFN‐γ MoAb had no significant effect. These results indicate that IL‐4 and IFN‐γ have inhibitory effects on IL‐3‐and IL‐5‐induced eosinophil differentiation from its progenitor cells.