Open AccessReduced antigen‐presenting function of human Epstein‐Barr virus (EBV)‐B cells and monocytes after UVB radiation is accompanied by decreased expression of B7, intercellular adhesion molecule‐1 (ICAM‐1) and LFA‐3
Author(s) -
KREMER I. B.,
BOS J. D.,
TEUNISSEN M. B. M.
Publication year - 1995
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1995.tb03135.x
Subject(s) - lymphocyte function associated antigen 1 , antigen , cd19 , antigen presenting cell , immunology , cd40 , biology , cd14 , t cell , microbiology and biotechnology , cell adhesion molecule , intercellular adhesion molecule 1 , immune system , in vitro , cytotoxic t cell , biochemistry
SUMMARY In this study, the effect of ultraviolet‐B (UVB) radiation on antigen‐presenting function was studied, to investigate whether antigen‐presenting cells (APC) are inhibited by UVB through a common mechanism. Two types of human APC were used: EBV‐B cells and monocytes, and these were irradiated in vitro with single low doses of UVB (range 0–200 J/m 2 ). Irradiation of EBV‐B cells or monocytes resulted in similar dose‐dependent reduction in APC function, when determined by the allogeneic mixed leucocyte reaction (MLR) or Candida albicans ‐ or tetanus toxoid‐specific T cell response. Our study shows that the reduced APC function was not likely to be caused by alterations in antigen processing or cytokine production. However, UVB‐irradiated APC displayed marked changes in adhesion molecule expression. Irradiated EBV‐B cells showed reduced expression of ICAM‐1 (30%), LFA‐3 (25%) and B7‐1 (35%), white expression of HLA‐DR, CD19 and LFA‐1 was not affected. UVB irradiation of monocytes did result in reduction in the expression of HLA‐DR (30%), LFA‐3 (40%), ICAM‐1 (65%) and B7‐1 and B7‐3 (90%), but had no effect on CD14, LFA‐1 and ICAM‐3 expression. Addition of non‐irradiated cells (but not the supernatant of these cells) or CD28 antibodies partly restored T cell activation, indicating that UVB‐induced reduction in APC function is at least partly mediated via impairment of co‐stimulatory molecule expression.