Open AccessModulation of immune response to Lol p I by pretreatment with anti‐idiotypic antibody is not restricted to the idiotypic expression
Author(s) -
BOUTIN Y.,
HÉBERT J.
Publication year - 1994
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1994.tb06566.x
Subject(s) - idiotype , immunoglobulin idiotypes , immunology , antibody , immune system , immunoglobulin e , immunization , epitope , antigen , idiotopes , biology , monoclonal antibody
SUMMARY To study the role of anti‐idiotypic antibodies in the regulation of the immune response to Lol p I (the major allergenic component of rye grass pollen), we have recently generated a panel of three MoAbs directed against distinct epitopes of Lol p I and an anti‐idiotypic MoAb directed against the idiotype borne by one of the anti‐ Lol p I MoAbs (290A‐167). The effects of pretreatment with this anti‐idiotypic MoAb in BALB/c mice before immunization with the antigen have been examined. The anti‐idiotypic MoAb or unrelated MoAb were given weekly for 8 weeks intraperitoneally. Mice then received the antigen (2 μg) adsorbed with alum (2 mg) at weeks 9, 11 and 13. Serum anti‐ Lol p I antibodies (IgG or IgE) and specific idiotypic responses were measured. Anti‐ Lol p I IgG antibodies could be detected before immunization with Lol p I only in mice pretreated with anti‐idiotypic MoAb. Immunization with Lol p I induced an anti‐ Lol p I IgG response in both groups, but this response was higher in mice that received anti‐idiotypic MoAb. Similar profiles were seen for specific IgE antibodies and idiotypic responses. Surprisingly, idiotypes borne by other anti‐ Lol p I MoAbs (539A‐6 and 348A‐6) had also been enhanced after pretreatment with the anti‐290A‐167 MoAb. These observations suggested that the pretreatment with this anti‐idiotypic MoAb modulates not only the expression of the respective idiotype, but also affects other idiotype responses.