Open AccessA high percentage of HLA‐DQ + and HLA‐DR + mononuclear cells is associated with a low incidence of acute graft‐ versus ‐host disease after allogeneic bone marrow transplantation (BMT) in children
Author(s) -
GARIN L.,
RIGAL D.,
MARSAFY S.,
BERNAUD J.,
PHILIPPE N.,
SOUILLET G.
Publication year - 1994
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1994.tb06565.x
Subject(s) - peripheral blood mononuclear cell , immunology , human leukocyte antigen , hla dr , medicine , hla dq , transplantation , bone marrow , antigen , biology , in vitro , allele , biochemistry , gene , haplotype
SUMMARY In order to discover some biological markers of acute graft‐ versus ‐host disease (aGVHD), we have studied the percentage of peripheral monocytes and T lymphocytes bearing HLA‐DR and HLA‐DQ class II molecules. This study included 25 aliogeneic BMT in children, cither with ( n = 10) or without ( n = 15) aGVHD. Within 2 months after transplantation, a higher percentage of DQ + and DR + monocytes and of DQ + T lymphocytes was observed in patients without aGVHD compared with patients with aGVHD. The most discriminating marker was the strong increase in the percentage of DO + monocytes in patients without aGVHD ( P = 0·001). In a sequential study, we observed a low percentage of DQ + and DR + peripheral blood mononuclear cells (PBMC) as long as the clinical manifestations of aGVHD continued. We speculate if the modulation of DQ and DR molecules on PBMC after BMT is a consequence of the action of some lymphokines, and if it plays a role in the regulation of the acute GVH reaction. We conclude that MHC class II molecules on peripheral mononuclear cells may be reliable biological markers for the diagnosis of aGVHD.