Open AccessEffects of tumour necrosis factor‐alpha (TNF‐α), IL‐1β and monocytes on lymphokine‐activated killer (LAK) induction from natural killer (NK) cells and T lymphocytes
Author(s) -
YONEDA K.,
OSAKI T.,
YAMAMOTO T.,
UETA E.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb07971.x
Subject(s) - lymphokine activated killer cell , tumor necrosis factor alpha , immunology , cytokine , interleukin 12 , lymphokine , interleukin 2 , natural killer t cell , biology , interleukin 21 , cytotoxic t cell , immune system , t cell , in vitro , biochemistry
SUMMARY Roles of monocytes and cytokines were investigated on LAK induction from T and NK cells. Monocytes augmented more T‐LAK induction than did NK‐LAK. Expression of IL‐β, TNF‐α and interferon‐gamma (IFN‐γ)‐mRNA and their cytokine production were superior in NK cells compared with T cells in parallel with their LAK activities. An increase of TNF‐α, IL‐1β and IFN‐γ production was induced by co‐culturing NK or T cells with autologous monocytes. The augmentation of T cell cytokine production and T‐LAK activity by monocytes was more prominent than that of NK cells. TNF‐α and IL‐1β were generated 24 h after IL‐2 stimulation, and these cytokines were able to almost substitute for monocytes in LAK induction. Conversely, LAK induction was almost completely suppressed by both anti‐IL‐1β and anti‐TNF‐α antibodies, if they were added within 24 h after the start of the LAK induction. IFN‐γ, which was produced at a later stage, scarcely affected LAK induction in spite of the cooperation with TNF‐α. The results obtained indicate conclusively that the superiority of NK‐LAK depends on their superior productivity of both IL‐1β and TNF‐α, and that the up‐regulation of LAK induction by monocytes is largely due to the enhanced generation of both cytokines.