Open AccessIL‐5 in post‐traumatic eosinophilic pleural effusion
Author(s) -
SCHANDENÉ L.,
NAMIAS B.,
CRUSIAUX A.,
LYBIN M.,
DEVOS R.,
VELU T.,
CAPEL P.,
BELLENS R.,
GOLDMAN M.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb06506.x
Subject(s) - peripheral blood mononuclear cell , pleural cavity , eosinophilic , eosinophilia , pleural effusion , pleural disease , medicine , pneumothorax , pathology , pleural fluid , immunology , eosinophil , pleurisy , respiratory disease , biology , in vitro , lung , asthma , surgery , biochemistry
SUMMARY Thoracic trauma or pneumothorax can result in pleural fluid eosinophilia. In this study we investigated the role of the eosinophilopoietic cytokine IL‐5 in three cases of post‐traumatic eosinophilic pleural effusions (EPE). Using a specific immunoenzymatic assay, significant levels of IL‐5 were found in EPE (range 100–3000 pg/ml), while IL‐5 was undetectable (<25 pg/ml) in corresponding serum samples and in non‐eosinophilic pleural fluids. IL‐5 present in pleural fluids was found bioactive in a proiiferative assay using a mouse CTLL‐2 cell line transfected with the cDNA corresponding to the a chain of the human IL‐5 receptor. Using a reverse polymerase chain reaction (PCR) method, we found IL‐5 mRNA expression within pleural mononuclear cells from patients with EPE, but not in corresponding peripheral blood mononuclear cells (PBMC), confirming that IL‐5 is synthesized locally in the pleural cavity. In the two cases in which pleural CD4+ cells were purified, these cells were identified as the major source of IL‐5. Taken together, these data indicate that the development of post‐traumatic EPE is related to a local secretion of IL‐5 by CD4+ cells present in the pleural cavity.