Open AccessTransforming growth factor‐beta (TGF‐β) in human milk
Author(s) -
SAITO S.,
YOSHIDA M.,
ICHIJO M.,
ISHIZAKA S.,
TSUJH T.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb06004.x
Subject(s) - beta (programming language) , transforming growth factor beta , colostrum , transforming growth factor , tgf beta signaling pathway , biology , antibody , chemistry , endocrinology , microbiology and biotechnology , medicine , immunology , computer science , programming language
SUMMARY The amount of TGF‐β contained in human whey was studied by the colony formation of NRK47F cells. It was noted that a factor inducing colony formation did exist in human whey, and its action was neutralized when anti‐TGF‐β antibodies were introduced. This suggests that TGF‐β does exist in human whey. In colostrum, the total amount ofTGF‐β was 1365.7 ± 242.9 ng /ml, of which the active form comprised 728.1 ± 248.7 ng/ml (n=2l). In late milk, the total TGF‐β was 952.5 ± 212.6 ng/ml, with an active form of 178.7 ± 157.3 ng/ml. Thus human milk contains a large amount of active TGF‐β. Furthermore, it was revealed by the reverse transcriptase polymerase chain reaction that mRNAs coding TGF‐β 1 and TGF‐β 2 exist in human milk cells. These results suggest that both TGF‐β 1 and TGF‐β 2 exist in human milk.