Open AccessPhenotypic analysis of cells in bronchoalveolar lavage fluid and peripheral blood of maedi visna‐infected sheep
Author(s) -
LUJÁN L.,
BEGARA I.,
COLLIE D. D. S.,
WATT N. J.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb05894.x
Subject(s) - bronchoalveolar lavage , cd8 , immunology , lymphocyte , biology , mhc class ii , pathogenesis , cd5 , immune system , medicine , antibody , major histocompatibility complex , lung
SUMMARY A phenolypic analysis of bronchoalveolar lavage fluid (BALF) and peripheral blood (PB) cells in maedi visna virus (MVVVinlecled sheep has been performed. The differential cell eount in BALF from MVV‐infeeted animals was characterized by a significant increase ( P < 0±05) i n lymphocytes and neutrophils. Lymphocyte phenotyping in BALF from MVV‐infeeted sheep showed a significant decrease ( P < 0·05) or CD4 + cells, a significant increase ( P < 0·05) of CD8 + eells and a significant inversion ( P <0·001) of the CD4 + /CD8 + ratio. CD5 + lymphocytes were also significantly decreased ( P < 0·05). γδ T cells and B cells did not differ significantly when compared with the controls. No correlation was observed between BALF and PB lymphocyte phenotypes. BALF macrophages from MVV‐infeeted animals showed inereased MHC class II expression and BALF lymphocytes from the same animals demonstrated up‐regulation of LFA‐1 and LFA‐3 expression. These findings and their relationship with lentiviral pathogenesis are discussed.