Open AccessRo/SSA inhibits the autologous mixed lymphocyte reaction
Author(s) -
KARSH J.,
HARLEY J. B.,
GOLDSTEIN R.,
LAZAROVITS A. I.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb03362.x
Subject(s) - phytohaemagglutinin , pokeweed mitogen , mixed lymphocyte reaction , immunology , lymphocyte , immune system , t lymphocyte , in vitro , biology , antigen , inducer , t cell , microbiology and biotechnology , andrology , medicine , concanavalin a , biochemistry , gene
SUMMARY To test the hypothesis that the Ro/SSA autoantigen can be recognized as antigenic by the human immune system, lymphocytes obtained from normal volunteers were used in in vitro assays evaluating the ability of Ro/SSA (mol. wt 60 kD) to induce B and/or T cell responses. Bovine Ro/SSA strongly inhibited the autologous mixed lymphocyte reaction in a dose‐dependent manner without similar effects on concurrently performed allogeneic mixed lymphocyte reactions or T cell proliferation induced by phytohaemagglutinin. Using three colour FACS analysis, Ro/SSA was found to decrease the percentage of CD4 + CD45 + RA + T cells in the proliferative, S+(G 2 + M), phase of the cell cycle. Associated with the decrease in the percentage of suppressor‐inducer cells, was the finding that Ro/ SSA was able to augment RF production in pokeweed mitogen stimulated cultures of peripheral blood lymphocytes.