Open AccessSynovial fluid cells in juvenile arthritis: evidence of selective T cell migration to inflamed tissue
Author(s) -
SILVERMAN E. D.,
ISACOVICS B.,
PETSCHE D.,
LAXER R. M.
Publication year - 1993
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1993.tb03360.x
Subject(s) - synovial fluid , il 2 receptor , peripheral blood mononuclear cell , immunology , cd8 , cd3 , t cell , synovitis , arthritis , medicine , biology , pathology , immune system , in vitro , biochemistry , alternative medicine , osteoarthritis
SUMMARY The perpetuation of chronic synovitis in juvenile arthritis (JA) is a complex interaction of local and systemic regulatory mechanism. We examined the cell surface phenotypc of synovial fluid cells and peripheral blood lymphocytes from 15 patients with J A to better understand the mechanism of local inflammation. Synovial fluid and peripheral blood mononuclcar cells were analysed for cell surface expression of CD2, CD3, CD4, CD8, CD19, CD25, CD29, CD45R and Ia using flow cytometry. We found a very low percentage of B cells with a concomitant increase of T cells in synovial fluid as compared with peripheral blood. A large percentage of the synovial fluid T cells were HLA‐DR + .or activated T cells, and there was a relative decrease in CD4 + cells in synovial fluid as compared with peripheral blood. There was only a minimal increase in CD25 + synovial fluid cells. The synovial fluid CD4 + cells were mainly of the CD2 high . CD29 + , CD45RO phenotype. This CD4 phenotype found on synovial fluid cells from patients with JA and in particular the CD29 cell surface marker, which recognizes a common β‐chain of adhesion molecules, is associated with binding to extracellular matrix proteins and is also associated with “primed” T cells. Our results demonstrated the presence of T cells which either selectively migrate to synovium and synovial fluid or are activated in situ in the joint.