Open AccessMonocyte cytokine secretion induced by chemically‐defined derivatives of Klebsiella pneumoniae
Author(s) -
HMAMA Z.,
LINA G.,
VINCENT C.,
WIJDENES J.,
NORMIER G.,
BINZ H.,
REVILLARD J. P.
Publication year - 1992
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1992.tb06886.x
Subject(s) - lipopolysaccharide , monocyte , tumor necrosis factor alpha , cytokine , concanavalin a , biochemistry , secretion , chemistry , biology , in vitro , immunology
SUMMARY The capacity of a K. pneumoniae membrane proteoglycan (Kp‐MPG) and four of its chemically defined derivatives to activate human monocytes was studied by measuring immunoreactive IL‐1β, IL‐6 and tumour necrosis factor‐alpha (TNF‐α) in culture supernatants. Monocyte culture supernatants were also tested for their comitogenic activity on concanavalin A‐stimulated thymocytes and for their cytotoxic activity on the mouse fibroblastic L929 cell line. The four Kp‐MPG derivatives were: (i) an acylpoly(1‐3)galactoside (APG); (ii) an APG preparation submitted to acid hydrolysis which removed all fatty acids but left intact the galactose chain of APG (GC‐APG); (iii) a preparation obtained by mild alkaline hydrolysis, containing additional ester‐linked C 14 and C 16 fatty acids bound to the APG molecule (EFA‐APG); and (iv) a polymer of the latter compound (APG pol). Kp‐MPG induced the synthesis of IL‐1β. IL‐6 and TNF‐α with dose‐responses and kinetics similar to those of Salmonella minnesota lipopolysaccharide (Sm‐Re‐LPS). APG pol and EFA‐APG induced the secretion of the three cytokines with lower potency than Kp‐MPG or Sm‐Re‐LPS. APG did not trigger any detectable cytokine production and GC‐APG induced only borderline and inconsistent responses. Our data demonstrate the critical role of ester‐linked C 14 and C 16 fatty acids in the triggering of monocyte response to Kp‐MPG derivatives.