Open AccessInterferon‐alpha and dexamethasone inhibit adhesion of T cells to endothelial cells and synovial cells
Author(s) -
EGUCHI K.,
KAWAKAMI A.,
NAKASHIMA M.,
IDA H.,
SAKITO S.,
MATSUOKA N.,
TERADA K.,
SAKAI M.,
KAWABE Y.,
FUKUDA T.,
ISHIMARU T.,
KUROUJI K.,
FUJITA N.,
AOYAGI T.,
MAEDA K.,
NAGATAKI S.
Publication year - 1992
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1992.tb06470.x
Subject(s) - endothelial stem cell , interferon gamma , microbiology and biotechnology , cell adhesion molecule , biology , interleukin 21 , immunology , t cell , cytokine , in vitro , immune system , biochemistry
SUMMARY We investigated whether interferon‐gamma (IFN‐γ), interferon‐alpha (IFN‐α) and glucocorticoids affected the adhesion of T cells to human umbilical endothelial cells or human synovial cells. About 30% of peripheral blood T cells could bind to unstimulated endothelial cells, but only a few T cells could bind to unstimulaled synovial cells. When both endothelial cells and synovial cells were cultured with recombinant IFN‐γ (rlFN‐γ), the percentage of T cell binding to both types of cells increased in a dose‐dependent manner. rIFN‐α and dexamethasone blocked the T cell binding to unstimulated endothelial cells. Furthermore, rIFN‐α and dexamethasone suppressed T cell binding to both endothelial cells and synovial cells stimulated by IKN‐γ, and also inhibited intercellular adhesion molecule‐ l (ICAM‐1) expression on both endothelial cells and synovial cells stimulated by 1 FN‐γ. These results suggest that IFN‐α and glucocorticoids may inhibit T cell binding to endothelial cells or synovial cells by modulating adhesion molecule expression on these cells.