Open AccessHumanized monoclonal antibody CAMPATH‐1H: myeloma cell expression of genomic constructs, nucleotide sequence of cDNA constructs and comparison of effector mechanisms of myeloma and Chinese hamster ovary cell‐derived material
Author(s) -
CROWE J. S.,
HALL V. S.,
SMITH M. A.,
COOPER H. J.,
TITE J. P.
Publication year - 1992
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1992.tb06421.x
Subject(s) - chinese hamster ovary cell , biology , transfection , microbiology and biotechnology , monoclonal antibody , effector , cell culture , complementary dna , immunoglobulin light chain , cytotoxicity , antibody , cancer research , immunology , genetics , gene , in vitro
SUMMARY Expression of CAMPATH‐1H, a humanized MoAb directed against an abundant surface antigen on human lymphocytes, has been studied using transfected myeloma cells. As the site of chromosome integration of DNA transfected into a cell is random we investigated the feasibility and frequency of hitting a ‘jackpot’ site for expression after co‐transfection with CAMPATH‐1H heavy and light chain constructs in genomic context. A cell line generating 40 μg/ml of CAMPATH‐1H in spent culture supernatant was achieved. The full nucleotide sequence of the CAMPATH‐1H heavy and light chain cDNA clones is also shown, in addition a comparison of the effector mechanisms, antibody dependent cellular cytotoxicity and complement dependent cytotoxicity of myeloma cell and Chinese hamster ovary (CHO) cell‐derived CAMPATH‐1H is reported.