Open AccessGlomerular immune deposits in murine lupus models may contain histones
Author(s) -
SCHMIEDEKE T.,
STOECKL F.,
MULLER S.,
SUGISAKI Y.,
BATSFORD S.,
WOITAS R.,
VOGT A.
Publication year - 1992
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1992.tb05867.x
Subject(s) - lupus nephritis , histone , immunology , pathogenesis , biology , immune system , immunofluorescence , antibody , proteinuria , systemic lupus erythematosus , western blot , kidney , endocrinology , medicine , disease , genetics , gene
SUMMARY Two types of lupus mice, NZB/NZW F 1 female hybrids and mice with graft‐ rersus ‐host disease (GVHD). were studied. Histones H3 and H2A were detected by immunofluorcscenee in glomeruli of 22/22 proteinuric GVHD and 8/12 proteinuric NZB/W F 1 female mice; in non‐proteinuric animals. 3/5 GVHD and 2/27 NZB/W F 1 female were positive. Using antibodies to histone peptides it was shown that mainly the N‐terminal regions of histones H3 and H2A were exposed in glomerular deposits. Western blot analysis revealed antibodies lo histone subfractions in sera of 33/34 lupus mice that developed proteinuria. This study provides evidence that histones are involved in the pathogenesis of lupus nephritis.