Open AccessIdentification of a myeloperoxidase inhibitor from normal human serum
Author(s) -
YEA C. M.,
DULARAY B.,
ELSON C. J.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb08171.x
Subject(s) - myeloperoxidase , immunology , identification (biology) , biology , medicine , inflammation , botany
SUMMARY An inhibitor of myeloperoxidase (MPO) has been identified in normal human serum. Initial experiments confirmed that high levels of MPO inhibitory activity are present in sera and that the inhibitor did not act by interfering with the assay. Purification of the inhibitor activity by salt precipitation followed by ton exchange and affinity chromatography revealed the presence of a protein of 150 kD. The purified inhibitory activity displayed dose and lime dependency and was not associated with IgG or IgA. It is considered that human serum contains an inhibitor of extracellular MPO capable of protecting against hypohalous acid release in host tissues and that if inhibitor levels are reduced such protection may fail.