Open AccessA 19‐year‐old man with leucocyte adhesion deficiency. In vitro and in vivo studies of leucocyte function
Author(s) -
DAVIES K. A.,
TOOTHILL V. J.,
SAVILL J.,
HOTCHIN N.,
PETERS A. M.,
PEARSON J. D.,
HASLETT C.,
BURKE M.,
LAW S. K. A.,
MERCER N. F. G.,
WALPORT M. J.,
WEBSTER A. D. B.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb08153.x
Subject(s) - in vivo , immunology , in vitro , function (biology) , adhesion , biology , medicine , chemistry , microbiology and biotechnology , biochemistry , organic chemistry
SUMMARY We describe a male patient with leucocyte adhesion molecule deficiency (LAD) or moderate phenotype. Although diagnosis was made only 2 years before his death, the patient survived until 19 yean of age. This enabled us to perform a number of novel investigations, both in vivo and in vitro , relating to his leucocyte biology. Monocytes cultured in vitro matured into morphologically normal, phagocytically capable macrophages, which were able to recognize aged‘apoptotic’neutrophils. By injection of radiolabelled autologous neutrophils we demonstrated a prolonged nculrophil half‐life, but normal margination, de‐margination on exercise, and splenic pooling. Neutrophil adherence in vitro to vascular cndotnclium was normal. Histological examination of the patient's lungs at postmortem showed intravascular aggregation of polymorphonuclear leucocytes but a paucity of cells in the interstitium and alveolar spaces. These findings indicate that the peripheral blood leucocytosis commonly observed in these patients may be due to prolonged intravascular neutrophil survival, and suggest that CD11/18 molecules have an important role in facilitating neutrophil emigration from blood vessels at sites of inflammation.