Open AccessPeripheral blood and synovial fluid T cells differ in their response to alloantigens and recall antigens presented by dendritic cells
Author(s) -
STAGG A. J.,
HARDING B.,
HUGHES R. A.,
KEAT A.,
KNIGHT S. C.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb08126.x
Subject(s) - immunology , antigen , antigen presenting cell , t cell , population , cytotoxic t cell , synovial fluid , dendritic cell , cd40 , immune system , biology , medicine , in vitro , pathology , biochemistry , alternative medicine , environmental health , osteoarthritis
SUMMARY Properties of T cells from inflammatory lesions were analysed by comparing the response of peripheral blood (PB) and synovial fluid (SF)T cells from 19 patients with a range of arthropathies to enriched allogeneic dendritic cells (DC) in a primary mixed leucocyte reaction (MLR). In 17 patients the proliferative response of SF T cells was significantly ( P < 0.05) less than that of PB lymphocytes. The reduced response of SF T cells was observed in all disease categories studied and could not be attributed to differences in cell number requirements or response kinetics. Addition of recombinant interlcukin‐2 enhanced the response of SF T cells in a dose‐dependent manner. Cell mixing experiments suggested that active suppression was not the underlying mechanism of the poor MLR response of SF T cells. In contrast to the MLR response, SF T cells were able to mount vigorous proliferative responses to recall antigen presented by autologous antigen‐presenting cells. The possibility is discussed that T cells compartmentalized at inflammatory lesions are a unique population with a diminished ability to interact with DC and respond to primary stimuli but an ability to respond to secondary antigenic challenge.