Open AccessEnhanced serum levels of soluble HLA class I molecules are induced by treatment with recombinant interferon‐gamma (IFN‐ γ )
Author(s) -
AULITZKY W. E.,
GROSSEWILDE H.,
WESTHOFF U.,
TILG H.,
AULITZKY W.,
GASTL G.,
HEROLD M.,
HUBER C.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05802.x
Subject(s) - beta 2 microglobulin , human leukocyte antigen , recombinant dna , radioimmunoassay , immunology , beta (programming language) , medicine , interferon gamma , endocrinology , antigen , biology , cytokine , biochemistry , computer science , gene , programming language
SUMMARY In order to investigate serum levels of soluble HLA class I antigens after single injection of various doses of recombinant IFN‐ γ (rIFN‐ γ ) and to correlate the changes observed to beta‐2‐microglobulin serum levels, we studied five patients with metastasizing renal cell carcinoma. Each patient received three treatment cycles of 10, 100 and 500 μ g rIFN‐ γ three times at weekly intervals. The treatment cycles were separated by a therapy‐free interval of 2 weeks. The order of dose levels was randomly assigned to each patient. Serum levels of soluble HLA class I proteins were measured by an ELISA in samples drawn immediately before and 4, 24, 48, 72 and 168 h after each administration of rIFN‐ γ . Beta‐2‐microglobulin was assessed in parallel using a commercially available radioimmunoassay. Significant induction of soluble HLA class I protein serum levels was observed after treatment with 100 and 500 μg rIFN‐ γ . The increments peaked after 2–4 days and remained elevated for up to more than 7 days. A significant correlation between increments of soluble HLA class I proteins and beta‐2‐microglobulin was observed. We conclude that measurement of soluble HLA serum levels is practical for monitoring induction of HLA class I synthesis in patients treated with rlFN‐ γ . The correlation observed between induction of beta‐2‐microglobulin and soluble HLA class 1 proteins indicates that measurement of beta‐2‐microglobulin might be sufficient for the biological response monitoring in clinical studies.