Open AccessAberrant T‐regulation in rheumatoid arthritis and IgA nephropathy affects CD5 + and CD5 – B lymphocytes equally
Author(s) -
JONES B. M.,
CHENG I. K. P.,
WONG R. W. S.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05798.x
Subject(s) - cd5 , immunology , pokeweed mitogen , antibody , cd8 , peripheral blood mononuclear cell , medicine , immune system , b cell , biology , in vitro , biochemistry
SUMMARY T‐suppressor function and T‐helper function in healthy adults, elderly patients with non‐immune diseases, and patients with rheumatoid arthritis (RA) and IgA nephropathy (IgAN) were titrated by adding graded concentrations of CDS + cells to autologous CD8‐depleted peripheral blood mononuclear cells (PBMC), or CD4 + cells to CDX – 4 – PBMC, respectively. Following culture with pokeweed mitogen (PWM), numbers of CD5 + and CD5 – immunoglobulin‐secreting cells were determined using a combination of rosetting with anti‐CD5‐coated Dynabeads and reverse haemolytic plaque formation (Jones, 1990). Of 11 RA patients studied, eight had slightly reduced suppressor activity for CD5 + and CD5 – IgM‐secreting cells, and three with active disease and high serum levels of C‐reactive protein, could not suppress IgG, IgA or IgM secretion by either B subset. Helper activity for both CD5 + and CD5 – B cells was slightly but significantly increased in RA patients, One of eight patients with IgAN could not suppress IgG, IgA or IgM production by CD5 + or CD5 – B cells, and all IgAN patients required strikingly fewer CD4 + cells for PWM‐induced activation or CD5 + and CD 5 – B cells than controls. It was concluded that in two immunologically mediated diseases in which some patients have raised numbers of circulating CD5 + B cells, aberrant T‐regulation affects CD5 + and conventional CD5 – B cells equally.