Open AccessInduction of type II collagen‐specific antibody production in blood lymphocyte cultures of Rhesus monkeys ( Macaca mulatto ) with collagen‐induced arthritis using the immobilized native antigen
Author(s) -
HART* B. A. T,
BOTMAN C. A. D.,
BAKKER N. P. M.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05646.x
Subject(s) - antigen , immunology , peripheral blood mononuclear cell , antibody , epitope , arthritis , type ii collagen , lymphocyte , biology , chemistry , medicine , in vitro , biochemistry
SUMMARY Peripheral blood mononuclear cells (PBMC) from Rhesus monkeys previously immunized with bovine type II collagen to induce arthritis were cultured with the same antigen. Because the native protein is poorly soluble in culture medium a healing step is often used. The antigen in this form induced PBMC proliferation, but epitopes for the induction of antibody production and arthritis were lost. To keep the native protein intact it was coated on affigel beads. With the immobilized antigen specific antibody production could be induced.