Open AccessDifferential effects of CD45 CD45R and CD45R0 monoclonal antibodies in modulating human B cell activation
Author(s) -
DEANE D. L.,
HARVEY E.,
STEEL C. M.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb05610.x
Subject(s) - monoclonal antibody , immunology , antibody , antigen , biology , t cell , immune system
SUMMARY We have examined the effect of monoclonal antibodies (MoAbs) to different epitopes of the leucocyte common antigen (LCA). CD45, on anti‐human immunoglobutin‐primed B cell activation. Binding of MoAbs to restricted epitopes present on CD45 glycoproteins of 180 kD and 220 kD (designated CD45R0 and CD45R. respectively) was found to promote B cell proliferation in the presence of T cells. CD45 MoAbs reactive with ‘public’ determinants on all four constituent members of the LCA family (180, 190, 205, and 220 k D) had either little effect or inhibited the basal B cell response to anti‐immunoglobulin priming. Simultaneous immunonuorcsccnt analysts of 5‐broinodeoxyuridinc incorporation and the expression of CD19 (B cell specific) or CD2 (T cell specific) identified the majority of responder cells as B lymphocytes. CD45R MoAbs significantly enhanced the B cell response to sub‐optimal concentrations of interleukin‐2. CD45 and CD45R0 MoAbs failed to elicit a similar response. Antibody to the interleukin‐2 receptor (anti‐Tac) partially blocked the CD45R‐driven. T cell‐dependent B cell proliferation.