Open AccessAnti‐human thyroid peroxidase and anti‐human thyroglobulin antibodies present no cross‐reactivity on recombinant peptides
Author(s) -
HENRY M.,
ZANELLI E.,
MALTHIERY Y.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb02956.x
Subject(s) - polyclonal antibodies , thyroglobulin , thyroid peroxidase , epitope , antibody , recombinant dna , antigen , immunology , thyroid , biology , virology , microbiology and biotechnology , medicine , endocrinology , biochemistry , gene
SUMMARY Thyroglobulin (Tg) and thyroid peroxidase (TPO) are two antigens largely recognized by the sera from patients with autoimmune thyroid disease (AITD). Recently, the complete mapping of both antigens was established with rabbit polyclonal antibodies by the use of recombinant proteins expressed in prokaryotic vector. Several investigators have argued for the existence of a crossreactivity of some hetero‐ and autologous antibodies versus these two proteins. In the present study, using rabbit polyclonal antibody, mouse polyclonal antibody and autoimmune antibody (aAb). we observed no common epitope on human Tg (hTg) and human TPO (hTPO).