Open AccessT cell receptor γδ bearing cells are decreased in the peripheral blood of patients with atopic diseases
Author(s) -
SCHAUER U.,
DIPPEL E.,
GIELER U.,
BRÄUER J.,
JUNG T.,
HEYMANNS J.,
RIEGER C. H. L.
Publication year - 1991
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1991.tb02950.x
Subject(s) - t cell receptor , cd8 , immunology , delta , atopic dermatitis , cd3 , t lymphocyte , t cell , immunofluorescence , monoclonal antibody , medicine , biology , microbiology and biotechnology , antibody , antigen , immune system , engineering , aerospace engineering
SUMMARY The biological role of T cell receptor (TCR) γδ bearing cells is currently not fully understood. Recently, a monoclonal antibody (TCRδ1) reacting against the whole molecule became available which facilitates the direct analysis of TCR‐γδ + cells. We studied 11 children with atopic dermatitis, 20 children with atopic asthma, 18 adults with atopic dermatitis and 38 healthy age matched controls aged 4–51 years. Lymphocytes were isolated from heparinized peripheral blood and the proportion of TCR‐γδ + lymphocytes was determined by FACS analysis. Patients with atopic diseases yielded a significantly ( P <0.01) lower proportion of TCR‐γδ + cells compared with normal controls (median 4.8% versus 7.1%). The percentage of TCR‐γδ + cells showed an age‐dependent decline in both the patient group ( r =—0.49, P <0.01) and the control group ( r=– ‐0.40, P <0.01). In addition, the proportion of cells which expressed CD8, TCR‐γδ or CD4, TCR‐γδ simultaneously was determined by double labelling immunofluorescence. Whereas CD4 + , TCR‐γδ + cells could be identified in only a few individuals, CD8 + , TCR‐γδ + cells were found in nearly all controls (median 2.4%, range 0.0–10.8%); atopic patients displayed significantly ( P < 0.01) lower proportions of CD8+, TCR‐γδ+ cells.