Open AccessThe inductive effect of interleukin‐4 on IgG4 and IgE synthesis in human peripheral blood lymphocytes
Author(s) -
ISHIZAKA A.,
SAKIYAMA Y.,
NAKANISHI M.,
TOMIZAWA K.,
OSHIKA E.,
KOJIMA K.,
TAGUCHI Y.,
KANDIL E.,
MATSUMOTO S.
Publication year - 1990
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1990.tb08101.x
Subject(s) - immunology , immunoglobulin e , peripheral blood , peripheral , interleukin 5 , interleukin 4 , medicine , interleukin , biology , cytokine , antibody
SUMMARY Using murine monoclonal antibodies against human IgG subclasses, specific and sensitive ELISAs assay to quantify the four human IgG subclasses in cell culture supernaUints were established. The effect of human recombinant interleukin‐4 (IL‐4) on the regulation of IgG subclasses by normal peripheral blood lymphocytes was investigated. In addition to the enhancement of lgE synthesis. IL‐4 preferentially induced IgG4 synthesis in vitro , whereas IL‐4 had no effect on IgGl, IgG2, and IgG3 synthesis. IL‐4‐induced IgG4 production was blocked in a dose‐dependent manner by recombinant interferon‐gamma and anti‐human IL‐4 monoclonal antibody. Collectively, this data indicates that IL‐4 plays an important regulatory role in both IgG subclass and IgE synthesis.