Open AccessElevated binding activity of CD8 + cells with phytohaemagglutinin by asbestos fibre in vitro
Author(s) -
KINUGAWA K.,
HYODOH F.,
ANDOH A.,
UEKI A.,
TANAKA H.,
MOCHIZUKI Y.
Publication year - 1990
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1990.tb06446.x
Subject(s) - chrysotile , phytohaemagglutinin , in vitro , peripheral blood mononuclear cell , cd8 , biology , microbiology and biotechnology , immunology , chemistry , immune system , asbestos , biochemistry , materials science , metallurgy
SUMMARY Asbestos fibres are known to depress the mitogenic stimuli of phytohaemagglutinin (PHA) to lymphocytes. We examined effects of asbestos (chrysotile) fibre on the proliferation of PHA‐stimulated lymphocytes and the PHA binding activity of lymphocytes in vitro. The incorporation of 3 H‐thymidine and the expression of interleukin‐2 receptor were depressed when the cells were exposed to 50 μg/ml of chrysotile fibre. The PHA binding activity of lymphocytes was significantly enhanced after chrysotile fibre exposure, as compared with non‐exposed group. These results indicate that the depression of PHA stimuli with chrysotile fibre was not due to blocking of PHA binding to lymphocytes. The enhancement of PHA binding activity by chrysotile fibre was observed on CD8 + but not on CD4 + cells. It is possible, therefore, that treatment of peripheral blood mononuclear cells with chrysotile fibre induces increased reactivity of CDS + cells with PHA, and that intensely activated CD8 + cells suppress proliferation of lymphocytes.