Open AccessInduction of B cell responsiveness to growth factors by Epstein‐Barr virus conversion: comparison of endogenous factors and interleukin‐1
Author(s) -
BLAZAR B. A.,
MURPHY A. M.
Publication year - 1990
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1990.tb06442.x
Subject(s) - cell culture , epstein–barr virus , biology , b cell , lymphokine , virus , immunology , cell growth , lymphocyte , interleukin 4 , cytokine , virology , immune system , antibody , genetics
SUMMARY Immortalized B lymphocytes produce a factor(s) that stimulates growth of B cell lines carrying Epstein Barr virus (EBV). Stimulatory supernatants derived from B cells also exhibit interleukin‐1 (IL‐1) activity in costimulator assays with the D10.G4.1 helper T cell line. Experiments with purified macrophage‐derived IL‐1 and recombinant IL‐1β demonstrate that IL‐1 stimulates proliferation of the cell lines that respond to the factors from B lymphocyte lines. One B cell line, Ramos, an EBV Burkitt's lymphoma, contrasts with other B cell lines in that it is refractory to the growth enhancing effects of B cell conditioned medium and macrophage‐derived IL‐1. When EBV was introduced into Ramos cells, growth was enhanced by the factor(s) in B cell conditioned medium (six out of seven lines); growth of EBV‐converted Ramos lines (six out of seven lines) also was enhanced by IL‐1. These findings demonstrate that infection of a non‐responsive transformed B lymphocyte by EBV induces cellular responsiveness to factor‐mediated growth stimulation.