Open AccessEffects of interleukin‐2 and interleukin‐2‐activated cells on in vitro myelopoiesis
Author(s) -
CLERIGUE M.,
PISA P.,
TSAI L.,
HANSON M.
Publication year - 1990
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1990.tb05356.x
Subject(s) - cytotoxic t cell , lymphokine activated killer cell , myelopoiesis , haematopoiesis , interleukin 3 , immunology , interleukin 2 , lymphokine , cytokine , peripheral blood mononuclear cell , biology , tumor necrosis factor alpha , progenitor cell , population , interleukin 12 , in vitro , interleukin 21 , stem cell , immune system , medicine , microbiology and biotechnology , biochemistry , environmental health
SUMMARY Lymphokine‐activated killer (LAK) cells from human peripheral blood mononuclear cells cultured with recombinant interleukin‐2 (IL‐2) have been used clinically in adoptive immunotherapy for cancer patients. To study the influence of LAK cells and IL‐2 on haematopoiesis, an in vitro assay system for colony formation of granulocyte‐macrophage progenitor cells (GM‐CFC) was used. LAK cells from cultures of either human peripheral blood (PB) or human bone marrow (BM) mononuclear cells were both inhibitory to allogeneic BM‐derived GM‐CFC. Inhibitory activity could be transferred with supernatants from co‐cultures of LAK cells and BM targets, but also from the IL‐2 activated PB‐ or BM‐derived cells alone. The inhibitory activity from the initially non‐cytotoxic/non‐inhibitory BM population was rapidly induced by IL‐2 activation, and preceded the generation of cytotoxic LAK cells in the culture. These experiments show that inhibition of haematopoietic progenitor cells by IL‐2 is not dependent on generation of cytotoxic LAK cells, but rather the result of IL‐2‐induced cytokine production. We conclude that the synergistic action of interferon‐gamma (IFN‐γ) and tumour necrosis factor‐alpha (TNF‐α) may contribute to inhibition, but that also other cytokines are responsible for the observed inhibition of BM‐derived GM‐CFC.