Open AccessStimulation of FACS‐analysed CD4 + and CD8 + human tumour‐infiltrating lymphocytes with ionomycin + phorbol‐12, 13‐dibutyrate does not overcome their proliferative deficit
Author(s) -
STOECK M.,
MIESCHER S.,
QIAO L.,
CAPASSO P.,
BARRAS C.,
FLIEDNER V.
Publication year - 1990
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1990.tb05135.x
Subject(s) - ionomycin , phytohaemagglutinin , cd8 , immunology , lymphocyte , t lymphocyte , biology , phorbol , stimulation , microbiology and biotechnology , immune system , endocrinology , signal transduction , protein kinase c
SUMMARY Human tumour‐infiltrating lymphocytes (TIL) were prepared by enzyme digestion from a series of different tumours and were purified on a fluorescence‐activated cell sorter (FACS II) according to their CD4 + and CD8 + phenotype. CD4 + and CD8 + TIL were stimulated separately in a low density microculture system with phytohacmagglutinin (PHA) or with ionomycin plus phorbol‐12, 13‐dibutyrate (PDBu). The PHA‐induced proliferation of TIL was highly decreased when compared with control peripheral blood lymphocytes. A decreased proliferation of TIL was also observed when cells were stimulated with ionomycin plus PDBu, a combination which is thought to circumvent early events associated with lymphocyte activation. Some TIL were also plated in limiting dilution where they showed decreased frequencies of proliferating T cell precursors. The data suggest that one component of the inhibition of TIL must be acting ‘ownstream’ of the early events of lymphocyte activation.