Open AccessAutoantibodies to mitochondrial and centromere antigens in primary biliary cirrhosis and systemic sclerosis
Author(s) -
McHUGH N. J.,
JAMES I. E.,
FAIRBURN K.,
MADDISON P. J.
Publication year - 1990
Publication title -
clinical & experimental immunology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.329
H-Index - 135
eISSN - 1365-2249
pISSN - 0009-9104
DOI - 10.1111/j.1365-2249.1990.tb03325.x
Subject(s) - sclerodactyly , primary biliary cirrhosis , autoantibody , immunology , scleroderma (fungus) , medicine , serology , antigen , rheumatoid arthritis , antibody , biliary cirrhosis , pyruvate dehydrogenase complex , autoimmune disease , pathology , biology , enzyme , calcinosis , biochemistry , inoculation , calcification
SUMMARY High levels of IgG antibodies to the multi‐enzyme complex bovine pyruvate dehydrogenase (PDHC) were detected by a sensitive ELISA in all patients with histologically proven primary biliary cirrhosis (PBC), in a minority of patients with systemic sclerosis without overt PBC or with chronic liver diseases of another cause and in no rheumatoid arthritis or normal controls. Most (15/21) but not all of these positive patients recognized a 70‐kD (E2) component of PDHC on immunoblotting. Additional recognition of a centromere‐associated 140‐kD polypeptide was associated with the presence of scleroderma‐like features (Raynaud's phenomenon and sclerodactyly) in PBC, further emphasizing the serological and clinical overlap between these two conditions.