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Dickkopf‐1 inhibits the invasive activity of melanoma cells
Author(s) -
Chen J.,
Li H.,
Chen H.,
Hu D.,
Xing Q.,
Ren G.,
Luo X.
Publication year - 2012
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.2011.04276.x
Subject(s) - melanoma , wnt signaling pathway , immunohistochemistry , wound healing , cancer research , medicine , downregulation and upregulation , pathology , metastasis , cell migration , cell culture , biology , immunology , cancer , microbiology and biotechnology , signal transduction , gene , biochemistry , genetics
Summary Background. Malignant melanoma (MM) has the highest morbidity of any malignant tumour. It is known that the Wnt pathway of enhances the development of melanoma. Dickkopf (DKK)‐1, an inhibitor of the canonical Wnt/β–catenin pathway, might also inhibit the development of MM. Aim. To examine the influence of DKK‐1 expression on the invasive activity of MM cells. Methods. To detect DKK‐1 expression level we performed immunohistochemistry on human tissue sections of normal skin, malignant melanoma (MM) in situ , and MM with lymph‐node metastasis. To clarify the role that DDK‐1 plays in the invasive activity of MM cells, we performed a scratch‐wound healing assay and Transwell invasion assay for A375 cells infected with appropriate recombinant adenoviruses, then assessed the migration of these cells. Results. Fewer DDK1‐overexpressing A375 cells than control cells migrated to the scratch‐wound area or through the pores of the Transwell membrane to the lower wells. Downregulation of DKK‐1 in A375 cells had the opposite effect. Conclusions. In the present study, we found for the first time that DKK‐1 expression inhibits the invasive activity of MM cells.