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Ultraviolet B radiation to the eye induces pigmentation in the epidermis via the activation of the subunit gp91 phox of reduced nicotinamide adenine dinucleotide phosphate oxidase
Author(s) -
Hiramoto K.,
Sato E. F.
Publication year - 2012
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.2011.04149.x
Subject(s) - nicotinamide adenine dinucleotide phosphate , melanocyte , oxidase test , melanin , irradiation , microbiology and biotechnology , nadph oxidase , chemistry , melanocyte stimulating hormone , endocrinology , biology , biochemistry , hormone , reactive oxygen species , enzyme , cancer research , melanoma , physics , nuclear physics
Summary Irradiation by ultraviolet (UV)B is known to increase the number of dopamine (Dopa)‐positive melanocytes in the skin. In this study, a 2.5‐kJ/m 2 dose of UVB radiation was delivered by a sunlamp to the ear or the eye of wild‐type C57BL/6j mice and of gp91 phox −/− C57BL/6j mice that had a knockout mutation of the gp91 phox subunit of reduced nicotinamide adenine dinucleotide phosphate oxidase (NADPH). The degree of change in the Dopa‐positive melanocyte expression in was reduced in gp91 phox −/− mice given UVB irradiation to the eye, but not in those given irradiation to the ear. The plasma level of α‐melanocyte‐stimulating hormone (α‐MSH) in the blood increased in the C57BL/6j mice after irradiation to either the eye or the ear, but it did not increase in the gp91 phox −/− mice given UVB irradiation to the eye. Both gp91 phox and α‐MSH in the central nervous system seem to contribute to pigmentation after UVB irradiation of the eye in mice.