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Subungual keratoacanthoma: analysis of cell proliferation and copy number variation of oncogenes compared with periungual squamous cell carcinoma
Author(s) -
Honma M.,
Kato N.,
Hashimoto M.,
Takahashi H.,
IshidaYamamoto A.,
Iizuka H.
Publication year - 2011
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.2010.03841.x
Subject(s) - multiplex ligation dependent probe amplification , keratoacanthoma , basal cell , immunohistochemistry , pathology , epidermoid carcinoma , medicine , biology , gene , genetics , exon
Summary Background. Subungual keratoacanthoma (SUKA) is a rare cutaneous tumour with several features distinct from ordinary KA. SUKA may not show spontaneous regression and sometimes grows progressively, resulting in phalangeal bone destruction. This makes its distinction from digital squamous cell carcinoma (SCC) difficult. Aim. To investigate differences in molecular expression between SUKA and digital SCC. Methods. In addition to immunohistochemical analysis of Ki‐67, one of the markers differentiating KA from SCC, we investigated the copy numbers of various oncogenes by multiplex ligation‐dependent probe amplification (MLPA) using two cases of SUKA and three cases of periungual SCC. Results. Ki‐67 was moderately or strongly positive in SCC but negative in SUKA. The MLPA analysis showed that the nuclear factor ( NF ) κB1 and cortactin ( CTTN ; formerly known as EMS1 ) genes are amplified in SUKA but not in digital SCC. This increase in NFκB1 was confirmed by immunohistochemical analysis. Conclusion. NFκB1 could be a novel marker to differentiate between SUKA and SCC. Although this study was performed on limited numbers of patients with SUKA, MLPA analysis could be applied to differentiate other benign tumours from their malignant counterparts.