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Simple and rapid screening for HLA‐Cw*06 in Polish patients with psoriasis
Author(s) -
Rębała K.,
SzczerkowskaDobosz A.,
Niespodziana K.,
Wysocka J.
Publication year - 2010
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.2009.03627.x
Subject(s) - psoriasis , human leukocyte antigen , zygosity , allele , typing , locus (genetics) , polymerase chain reaction , immunology , medicine , antigen , biology , genetics , gene
Summary Background. The human leucocyte antigen (HLA) C allele Cw*06 is currently recognized as a major disease allele at the PSORS1 locus. It has been suggested that characterization of this gene could be used as a convenient criterion for classification of psoriasis phenotypes. Aim. To design and optimize a DNA typing procedure, suitable for identification of HLA‐Cw*06 and its zygosity status verification in large‐scale analyses, and to test for its robustness in a case–control study. Methods. PCR assays with sequence‐specific primers (PCR‐SSP) were used for specific detection of HLA‐Cw*06. PCR with analysis of restriction fragment length polymorphism was used to distinguish between patients homozygous and heterozygous for HLA‐Cw*06. Additionally, those homozygous for HLA‐Cw*06 were screened for nonspecific digestion by degenerated PCR‐SSP. This three‐step procedure was used in the examination of 383 patients with psoriasis that developed at the age of ≥ 30 years of age and of 143 healthy subjects from northern Poland. Results. A simple and rapid procedure for screening of HLA‐Cw*06 was produced. A significant difference in HLA‐Cw*06 frequency between patients with psoriasis and controls was seen ( P = 0.02). Detailed examination of the age of disease onset among patients with psoriasis revealed that involvement of HLA‐Cw*06 in the genetic background of psoriasis developing as late as the age of 45 years cannot be neglected. Conclusions. The low cost, high‐throughput capacity and requirement for small sample amounts make this procedure a useful one for HLA‐Cw*06 typing in clinical practice and large population studies. We recommend that patients with psoriasis diagnosed before 45 years of age should be considered for diagnostic HLA‐Cw*06 typing.