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Lymphocyte proliferation testing in chromium allergic contact dermatitis
Author(s) -
Martins L. E. A. M.,
Da Silva Duarte A. J.,
Aoki V.,
Nunes R. S.,
Ogusuku S.,
Reis V. M. S.
Publication year - 2008
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.2008.02725.x
Subject(s) - chromium , patch testing , contact dermatitis , allergic contact dermatitis , medicine , hand dermatitis , patch test , potassium dichromate , dermatology , sensitivity (control systems) , immunology , allergy , chemistry , metallurgy , materials science , inorganic chemistry , electronic engineering , engineering
Summary Background. Lymphocyte proliferation testing (LPT) has some advantages over patch testing to diagnose allergic contact dermatitis. It is harmless, objective and can be used in clinical situations where patch testing is not recommended. Unfortunately, significant success has only been achieved with nickel. There are few studies on chromium LPT and they were performed with different methods, leading to inconsistent results. Methods. To determine the best parameters for chromium LPT, we tested 20 patients with allergic contact dermatitis to the metal and 20 controls, using various protocols. Results. The best sensitivity and specificity ratios were achieved with 6‐day cultures stimulated with a range from 7.5 × 10 ‐4 to 5 × 10 ‐3 mol/L of nonfiltered chromium chloride solutions. The sensitivity, specificity and accuracy values found within this range were 65%, 95% and 80%, respectively. Conclusion. Further investigation is necessary to achieve better sensitivity values.