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Combined treatment with intratumoral injection of dendritic cells and topical application of imiquimod for murine melanoma
Author(s) -
Lee J.R.,
Shin J.H.,
Park J.H,
Song S.U.,
Choi G.S.
Publication year - 2007
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.2007.02453.x
Subject(s) - imiquimod , medicine , dermatology , melanoma , cancer research
Summary Background. The maturation state of dendritic cells is one of the factors that affect their capacity to induce antigen‐specific cytotoxic T lymphocytes. Topical cutaneous application of imiquimod can induce the maturation and migration of cutaneous dendritic cells. Objectives. To evaluate the synergistic effect of topical application of imiquimod plus intratumoral injection of syngeneic bone marrow‐derived dendritic cells in the treatment of melanoma. Methods. For the B16F10 melanoma model, naive C57BL/6 mice were inoculated intradermally with 2 × 10 3 B16F10 melanoma cells in the right upper flank. Four groups (untreated control, dendritic cells alone, imiquimod alone and imiquimod plus dendritic cells) were included in the animal study, with five mice in each group. Tumour size was measured every 2 weeks, and histochemical and immunohistochemical staining carried out. ELISpot and PKH assays were performed to assess immune activity. Results. Combined treatment of topical application of imiquimod and intratumoral injection of dendritic cells led to significant tumour regression, in contrast to partial eradication of the tumours with imiquimod or dendritic cells alone. Conclusion. These findings suggest that combination therapy with topical application of imiquimod and intratumoral administration of dendritic cells is a potent strategy for the treatment of melanoma.