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In vitro cytokine profile in childhood alopecia areata and the immunomodulatory effects of AS‐101
Author(s) -
Shohat M.,
Mimouni D.,
BenAmitai D.,
Sredni B.,
Sredni D.,
Shohat B.,
David M.
Publication year - 2005
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.2005.01817.x
Subject(s) - alopecia areata , phytohaemagglutinin , peripheral blood mononuclear cell , cytokine , medicine , immunology , interleukin 2 , in vitro , interleukin , receptor , endocrinology , interferon gamma , peripheral blood , chemistry , biochemistry
Summary The aim of the present study was to determine the effect of AS‐101, a known immunomodulator, on the pattern of cytokine production in children with patchy alopecia areata (PAA). Ten previously untreated children with PAA were compared to 10 healthy age‐ and sex‐matched controls. Peripheral blood mononuclear cells (PBMC) were isolated from all participants. Unstimulated and phytohaemagglutinin (PHA)‐stimulated PBMC were tested with and without the addition of AS‐101. The production of interferon gamma (IFNγ), soluble interleukin (IL)‐2 receptor (IL‐2R), IL‐10, IL‐5 and IL‐6 was determined. The levels of soluble IL‐2R, IL‐5 and IL‐6 were significantly higher in the PAA patients than the controls. AS‐101 inhibited the production of IL‐10, IFNγ, IL‐2R and IL‐5 in both PAA patients and controls, but there was a greater inhibitory effect in children with PAA.