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Lack of expression of the recombination activating genes RAG‐1 and RAG‐2 in cutaneous T‐cell lymphoma: pathogenic implications
Author(s) -
DÖBBELNG U.,
DUMMER R.,
SCHMID M.HESS,
BURG G.
Publication year - 1997
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1997.tb01074.x
Subject(s) - gene , lymphoma , genetics , recombination activating gene , biology , gene expression , cutaneous t cell lymphoma , expression (computer science) , cancer research , recombination , mycosis fungoides , immunology , computer science , programming language
Summary Analyses of the karyotype and genome of cutancous T‐cell lymphoma (CTCL) cells have shown that they contain chromosome breaks and translocations. The sequence analyses of such DNA breakpoints found in various kinds of leukaemias have suggested chat some of the observed translocations have been caused by illegitimate V(D)J (v = Variability; D = diversity; J = joining) recombination. To study whether illegitimate Y(D)J recombination is responsible for the continuously increasing number of DNA breaks in CTCL, we used reverse transcriptase polymerase chain reaction (RT‐PCR) to analyse three CTCL cell lines and biopsies from 14 CTCI. patients for the expression of the RAG‐1 and RAG‐2 genes which are essential for V(D)J recombination, We found no RAG gene expression in any of the 17 samples analysed, indicating that illegitimate V(D)J recombination may not be the reason for the increased number of chromosomal aberrations and trans‐locations in CTCL cells.