Premium
Analyses of T‐cell receptor β–chain genes by Southern blotting in known and suspected cutaneous T–cell lymphoma. A study of 67 samples from 32 patients
Author(s) -
WOLFF–SNEEDORFF A.,
RALFKIAER E.,
THOMSEN K.,
VEJLSGAARD G. L.
Publication year - 1995
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1995.tb02667.x
Subject(s) - mycosis fungoides , gene rearrangement , t cell receptor , lymphoma , cutaneous t cell lymphoma , lymph node , pathology , peripheral t cell lymphoma , southern blot , bone marrow , skin biopsy , biopsy , medicine , t cell , biology , gene , immunology , genetics , immune system
Summary In this study we have investigated the configuration of the T–cell receptor (TCR) β–chain genes in benign cutaneous conditions ( n = 5) and known ( n = 22) or suspected ( n = 5) cutaneous T–cell lymphoma (CTCL). Sequential biopsies from skin, lymph node, blood and/or bone marrow were available in 12 cases of the 22 confirmed CTCL, and a total of 67 samples were analysed. In the benign conditions, clonal rearrangements of the TCR β–chain genes were seen in neither skin nor blood samples. In contrast, in CTCL clonal rearrangements were detected in all skin samples from plaque or tumour lesions of mycosis fungoides. Clonal TCR rearrangements were also present in skin and blood samples from two patients with Sèzary's syndrome, and in skin and blood samples from three of five patients with clinically suspected CTCL. In 10 patients with large cell lymphomas, clonal rearrangements were detected in skin samples in half of the cases. In the remaining patients, clonal TCR rearrangements could not be detected in the skin, but only in the blood and/or bone marrow specimens. Results from the analyses of sequential biopsies showed identical patterns of rearrangement in 11 patients. In the remaining patient, the pattern of rearrangement differed between skin and lymph node. These data confirm and extend previous reports and indicate that analysis of TCR β–chain genes by Southern blotting forms a useful supplement to other methods for the diagnosis of known and suspected CTCL. They also emphasize the importance of studying not only skin, but also extracutaneous sites.