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The growth of ichthyotic epidermal cells in a 3‐dimensional reconstruction of human skin, the skin equivalent
Author(s) -
FEATHERSTONE J.,
DYKES P.J.,
MARKS R.
Publication year - 1995
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1995.tb01332.x
Subject(s) - human skin , skin equivalent , dermatology , medicine , cancer research , biology , keratinocyte , cell culture , genetics
Summary We have studied the differentiation of ichthyotic epidermis in vitro using the skin equivalent model. The morphology of these ichthyotic cultures has been investigated using histopathological and histometric techniques including epidermal and stratum corneum thickness measurements. The skin equivalents have also been investigated for the presence of markers of differentiation using immunolocalization techniques. These markers include the 65·5 and 67 kDa keratins, desmoplakin, involuerin, laminin and filaggrin. It has been shown that the ichthyotic epidermis develops a fully differentiated epidermis and stratum corneum, equivalent to those seen in normal skin equivalents.