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Effects of colchicine on the induction of ornithine decarboxylase and its gene expression by the phorbol ester tumour promoter
Author(s) -
KONO T.,
TANIGUCHI S.,
MIZUNO N.,
FUKUDA M.,
MAEKAWA N.,
HISA T.,
ISHII M.,
OTANI S.,
HAMADA T.
Publication year - 1993
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1993.tb02205.x
Subject(s) - ornithine decarboxylase , colchicine , gene expression , microbiology and biotechnology , phorbol ester , tumor promotion , protein kinase c , phorbol , chemistry , signal transduction , gene , biology , medicine , biochemistry , enzyme , carcinogenesis
Summary The activity and gene expression of ornithine decarboxylase (ODC, an indicator of tumour promotion) were induced by the phorbol ester tumour promoter, 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA), in mouse skin. In the present study, the effect of colchicine, a microtu‐bule‐disrupting agent, on ODC activity and its gene expression were investigated. On administration of colchicine (100 μg) intraperitoneally 1·5 h before TPA treatment, ODC activity and ODC mRNA levels stimulated by TPA were suppressed to about 52 and 64%, respectively. These results suggest the involvement of a microtubule or colchicine‐sensitive substrate in the signal transduction system for gene expression.