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Modulation of morphological differentiation of human endothelial cells in culture by the synthetic peptide YIGSR and cytochalasin B
Author(s) -
KUBOTA Y.,
MIZOGUCHI M.
Publication year - 1993
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1993.tb02177.x
Subject(s) - medicine , cytochalasin b , dermatology , biology , genetics , in vitro
Summary Matrigel is a solubilized tissue basement membrane gel extracted from the mouse Engelbreth–Holm–Swarm sarcoma. Cultures of human endothelia! cells could be induced to undergo extremely rapid morphological differentiation into capillary‐like structures by Matrigel. The present study demonstrates the following: (a) Matrigel‐induced tube formation by endothelial cells is laminin‐dependent and, particularly, the YIGSR peptide site in the B1 chain of laminin may be necessary for endothelial cell differentiation resulting in capillary formation; (b) endothelial cells may be preprogrammed for rapid differentiation into blood vessels and do not require DNA, RNA or protein synthesis in order to undergo tube formation on Matrigel; and (c) microfilament assembly and function is important for Matrigel‐induced tube formation.