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The inter‐relation between inflammation and epidermal proliferation in normal skin following epicutaneous application of leukotriene‐B 4 —an immunohistochemical study
Author(s) -
JONG E.M.G.J.,
ERP P.E.J.,
VLIJMEN I.M.J.J.,
KERKHOF P.C.M.
Publication year - 1992
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1992.tb00249.x
Subject(s) - epidermis (zoology) , inflammation , leukotriene b4 , dermis , peripheral blood mononuclear cell , pathology , immunohistochemistry , immunology , chemistry , biology , medicine , in vitro , anatomy , biochemistry
Summary Topical application of leukotriene‐B 4 (LTB 4 ) on normal skin has been used as an in‐vivo model to investigate cutaneous inflammation and epidermal proliferation, which are important phenomena in the pathogenesis of psoriasis. The aim of the present investigation is to further elucidate the interrelation between inflammation and epidermal proliferation, using specific monoclonal antibodies as markers for the different cell types involved. Aliquots of LTB 4 were applied on the upperarms of eight healthy volunteers. After LTB 4 ‐application, biopsies were taken at consecutive time intervals. On frozen sections, epidermal proliferation was assessed by K s 8.12‐(keratin 16) and Ki‐67‐binding (cycling cells), inflammation was characterized using anti‐elastase (PMN), T11 (T‐lymphocytes), pan‐B (B‐lymphocytes), WT 14 (CD14‐positive cells) and OKT 6 (Langerhans cells). New observations were that the density of CD 14‐positive cells was increased even at 8 h and decreased slightly at 72 h, A striking rearrangement of Langerhans cells was seen in close vicinity to intra‐epidermal accumulations of PMN. Remarkably an increased density of these cells in the dermis at 72 h was seen and a decrease in the epidermis. In line with previous studies, the accumulation of PMN reached a maximum 24 h after LTB 4 ‐challenge. The identity of the mononuclear infiltrate cells which have been reported 48–72 h after LTB 4 proved to be T‐lymphocytes. No B‐lymphocytes were observed. Ki‐67‐positive nuclei were maximally increased 72 h after LTB 4 ‐application, which implies that recruitment of cycling cells is of relevance for the LTB 4 ‐induced proliferation in vivo. The hyperproliferation‐related keratin 16 was expressed inconsistently in the suprabasal compartment. The present investigation demonstrates that the CD14 cells and Langerhans cells participate in acute LTB 4 induced inflammation.