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Detection of herpes simplex viral DNA in Kaposi's varicelliform eruption using in situ hybridization method
Author(s) -
FUKUDA M.,
KONO T.,
ISHII M.,
HAMADA T.
Publication year - 1991
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1991.tb01223.x
Subject(s) - in situ hybridization , herpes simplex virus , staining , dna , in situ , cytoplasm , virology , microbiology and biotechnology , hybridization probe , biology , virus , chemistry , gene , genetics , messenger rna , organic chemistry
Summary Herpes simplex viral DNA was detected in biopsy specimens obtained from four patients with Kaposi's varicelliform eruption using an in situ hybridization technique with btotinylated complementary DNA probes. Herpes simplex viral DNA was consistently found almost exclusively in the nuclei of giant cells and balloon cells in vesicles during day 2, 5 and 7 of the disease. In the nuclei, the viral DNA staining pattern was granular or agglomerate. No positive staining was observed in the cytoplasm.