Premium
In vitro and in vivo phototoxicity of furocoumarin‐containing plants
Author(s) -
KNUDSON E.A.,
KROON S.
Publication year - 1988
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1988.tb00666.x
Subject(s) - phototoxicity , furocoumarins , erythema , furocoumarin , psoralen , in vivo , furanocoumarin , chemistry , botany , dermatology , in vitro , biology , horticulture , medicine , biochemistry , photochemistry , microbiology and biotechnology , dna
Summary The purpose of the study was to investigate the individual phototoxic reactivity in the skin to a series of plants, selected for their phototoxicity as determined in the Daniel's in vitro test. Twenty volunteers of skin type II, nine females and eleven males with normal sunlight reactivity were included in the study. Juice and leaves of wild parsnip, giant hogweed, angelica, garden parsley, celeriac and dill were applied on unpigmented skin of the thighs and irradiated after 1–11/2 h with 2 and 4 J/cm 2 UVA. The study showed clear ranking of the tested plants with wild parsnip and giant hogweed as the most and celeriac and dill as the least potent. Doubling of the UVA dose resulted in more and stronger erythema reactions. Hydration (occlusion) of the test substances had a similar promotion effect. Tests with leaves gave stronger reactions than those with juices. Isolated pigment reactions were often seen when the combined doses of furocoumarins and UVA were too weak to cause erythema. These reactions could be compared with Berloque dermatitis. Such pigment reactions are easily overlooked and could be one of the explanations for the few observed cases of phytophotodermatitis in the dcrmatological clinic.